LOCUS       sequence-124567-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of two tandem copies of the non-aggregating 
            delta-FG mutant, fused to the VP64 transcription activator, in 
            mammalian cells, under a UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pUBC_HA_2xPP7-VP64
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             173..193
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             197..217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             221..247
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             221..247
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
       61 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacc
      121 tggaggtacg gccaccgcca ccatggaccc taaaaaaaag agaaaggtgg acccaaagaa
      181 gaaacgcaaa gttgatccga agaagaaaag gaaagttggc tacccctacg acgtgcccga
      241 ctacgccgga agtggtgccg gaacaggagg ctccatgtca aagacgatcg tgctgtctgt
      301 aggtgaagcc acacgcaccc tcactgagat acagtccacg gccgataggc agatttttga
      361 ggagaaggtg ggtccactgg tgggtaggct tcgactgacg gcctccctga ggcaaaacgg
      421 cgctaaaacg gcttatcgcg ttaacctcaa gctggaccag gccgatgtcg ttgatagtgg
      481 actgcccaaa gtcaggtaca cacaggtgtg gagccacgat gtgaccatcg tggctaactc
      541 aacagaggcg tcccggaagt ctctgtacga cctgacaaag tccctggttg caacctctca
      601 ggtggaggat ctggtggtta atctcgtgcc cctcggacgg ggttccacga gcgggtcagg
      661 tggcggctcc atgtcaaaga cgatcgtgct gtctgtaggt gaagccacac gcaccctcac
      721 tgagatacag tccacggccg ataggcagat ttttgaggag aaggtgggtc cactggtggg
      781 taggcttcga ctgacggcct ccctgaggca aaacggcgct aaaacggctt atcgcgttaa
      841 cctcaagctg gaccaggccg atgtcgttga tagtggactg cccaaagtca ggtacacaca
      901 ggtgtggagc cacgatgtga ccatcgtggc taactcaaca gaggcgtccc ggaagtctct
      961 gtacgacctg acaaagtccc tggttgccac ctctcagg
//
