LOCUS       sequence-124568-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of two tandem copies of the non-aggregating 
            delta-FG mutant, fused to the VP64 transcription activator, in 
            mammalian cells, under a UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pUBC_HA_2xPP7-VP64
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..209)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 ataaacaact gatcagcggg tttaaacggg ccctcattta ttcaggtacc tcagttaatc
       61 agcatgtcca ggtcgaaatc atcaagggcg tcactgccga gcatgtcgag gtcaaagtca
      121 tcaagggcat ccgaaccaag catgtcaagg tcaaaatcat cgagggcgtc acttcccagc
      181 atatccagat caaaatcgtc caatgcgtca gcccgtccgg aaccggatcc accggaaccc
      241 cgtccgaggg gcacgagatt aaccaccaga tcctccacct gagaggtggc aaccagggac
      301 tttgtcaggt cgtacagaga cttccgggac gcctctgttg agttagccac gatggtcaca
      361 tcgtggctcc acacctgtgt gtacctgact ttgggcagtc cactatcaac gacatcggcc
      421 tggtccagct tgaggttaac gcgataagcc gttttagcgc cgttttgcct cagggaggcc
      481 gtcagtcgaa gcctacccac cagtggaccc accttctcct caaaaatctg cctatcggcc
      541 gtggactgta tctcagtgag ggtgcgtgtg gcttcaccta cagacagcac gatcgtcttt
      601 gacatggagc cgccacctga cccgctcgtg gaaccccgtc cgaggggcac gagattaacc
      661 accagatcct ccacctgaga ggttgcaacc agggactttg tcaggtcgta cagagacttc
      721 cgggacgcct ctgttgagtt agccacgatg gtcacatcgt ggctccacac ctgtgtgtac
      781 ctgactttgg gcagtccact atcaacgaca tcggcctggt ccagcttgag gttaacgcga
      841 taagccgttt tagcgccgtt ttgcctcagg gaggccgtca gtcgaagcct acccaccagt
      901 ggacccacct tctcctcaaa aatctgccta tcggccgtgg actgtatctc agtgagggtg
      961 cgtgtggctt cacctacaga cagcacgatc gtctttgaca tggagcctcc tgttccggca
     1021 ccacttccgg cgtagtcggg
//
