LOCUS       sequence-124582-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of humanized MS2 (V75E/A81G), fused to the VP64
            transcrption activator, in mammalian cells, under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV_mCherry_2A_HA_2xMS2-VP64
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..132
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             139..165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             139..165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             985..1005
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacgagcgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagggcagc
       61 accagcggcg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
      121 aatcctggcc caggcagtta cccctacgac gtgcccgact acgccggatc cggcgccggt
      181 accagcaatt tcactcaatt tgtgctcgtg gataacggag gaacgggcga cgtgaccgtg
      241 gcaccaagca actttgctaa tggagtggcc gagtggatta gctcaaatag caggtcccag
      301 gcttacaagg tcacctgtag tgtccggcaa tcatccgccc agaatcggaa atatactatt
      361 aaggtagaag tgccgaaagt ggcaactcaa accgtggggg gcgaggaatt gcctgtggcc
      421 ggctggagaa gctacctcaa catggaactc accattccaa tcttcgccac aaacagcgac
      481 tgtgagctca tcgtcaaagc catgcagggt ctgctgaagg acggcaatcc tattccatct
      541 gcaatcgcgg ccaattccgg catttatggt tccacgagcg ggtcagggag caatttcact
      601 caatttgtgc tcgtggataa cggaggaacg ggcgacgtga ccgtggcacc aagcaacttt
      661 gctaatggag tggccgagtg gattagctca aatagcaggt cccaggctta caaggtcacc
      721 tgtagtgtcc ggcaatcatc cgcccagaat cggaaatata ctattaaggt agaagtgccg
      781 aaagtggcaa ctcaaaccgt ggggggcgag gaattgcctg tggccggctg gagaagctac
      841 ctcaacatgg aactcaccat tccaatcttc gccacaaaca gcgactgtga gctcatcgtc
      901 aaagccatgc agggtctgct gaaggacggc aatcctattc catctgcaat cgcggccaat
      961 tccggcattt atggttccgg ggaccccaag aagaagagaa aggtggaggc cagcggttcc
     1021 ggacgggctg acgcattgga cgattttgat ctggatatgc tgggaagtga cgccctcgat
     1081 gattttgacc ttgacatgct tggttcggat gcccttgatg actttgacct c
//
