LOCUS       sequence-124583-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of humanized MS2 (V75E/A81G), fused to the VP64
            transcrption activator, in mammalian cells, under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV_mCherry_2A_HA_2xMS2-VP64
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..207)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(232..252)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctgcaata aacaactgat cagcgggttt aaacgggccc tcatttattc agttaatcag
       61 catgtccagg tcgaaatcat caagggcgtc actgccgagc atgtcgaggt caaagtcatc
      121 aagggcatcc gaaccaagca tgtcaaggtc aaaatcatcg agggcgtcac ttcccagcat
      181 atccagatca aaatcgtcca atgcgtcagc ccgtccggaa ccgctggcct ccacctttct
      241 cttcttcttg gggtccccgg aaccataaat gccggaattg gccgcgattg cagatggaat
      301 aggattgccg tccttcagca gaccctgcat ggctttgacg atgagctcac agtcgctgtt
      361 tgtggcgaag attggaatgg tgagttccat gttgaggtag cttctccagc cggccacagg
      421 caattcctcg ccccccacgg tttgagttgc cactttcggc acttctacct taatagtata
      481 tttccgattc tgggcggatg attgccggac actacaggtg accttgtaag cctgggacct
      541 gctatttgag ctaatccact cggccactcc attagcaaag ttgcttggtg ccacggtcac
      601 gtcgcccgtt cctccgttat ccacgagcac aaattgagtg aaattgctcc ctgacccgct
      661 cgtggaacca taaatgccgg aattggccgc gattgcagat ggaataggat tgccgtcctt
      721 cagcagaccc tgcatggctt tgacgatgag ctcacagtcg ctgtttgtgg cgaagattgg
      781 aatggtgagt tccatgttga ggtagcttct ccagccggcc acaggcaatt cctcgccccc
      841 cacggtttga gttgccactt tcggcacttc taccttaata gtatatttcc gattctgggc
      901 ggatgattgc cggacactac aggtgacctt gtaagcctgg gacctgctat ttgagctaat
      961 ccactcggcc actccattag caaagttgct tggtgccacg gtcacgtcgc ccgttcctcc
     1021 gttatccacg agcacaaatt gagtgaaatt g
//
