LOCUS       sequence-124592-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of humanized A. fulgidus L7Ae, fused to the 
            VP64 transcription activator, in mammalian cells, under a UBC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pUBC_HA_L7Ae-VP64
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             151..171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             175..195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             199..219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             661..810
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     primer_bind     complement(896..915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     950..969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1025..1041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1025..1041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1038..1060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
       61 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
      121 cctggaggta cggccaccgc caccatggac cctaaaaaaa agagaaaggt ggacccaaag
      181 aagaaacgca aagttgatcc gaagaagaaa aggaaagttg gctaccccta cgacgtgccc
      241 gactacgccg gaagtggtgc cggcaccggc ggcatgtacg tgcgcttcga ggtgcccgag
      301 gacatgcaga acgaggccct gagcctgctg gaaaaagtgc gcgagagcgg caaagtgaag
      361 aagggcacca acgaaaccac caaggccgtg gaacggggcc tggccaagct ggtgtatatc
      421 gccgaggacg tggacccccc cgagattgtg gcccatctgc ccctgctgtg cgaagagaag
      481 aacgtgccct acatctacgt gaagtccaag aacgacctgg gcagagccgt gggcatcgag
      541 gtgccatgtg cctctgccgc catcatcaac gagggcgagc tgcggaaaga actgggcagc
      601 ctggtggaaa agatcaaggg cctgcagaag ggttccggtg gatccggttc cggacgggct
      661 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
      721 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
      781 gcccttgatg atttcgacct ggacatgctg attaactgag gtacctgaat aaatgagggc
      841 ccgtttaaac ccgctgatca gttgtttatt gcagcttata atggttacaa ataaagcaat
      901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      961 aaactcatca atgtatctta tcgactgttt aaacctgcag gcatgcaagc ttggcgtaat
     1021 catggtcata gctgtttcct gtgtgaaatt gttatccgct caca
//
