LOCUS       sequence-124593-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of humanized A. fulgidus L7Ae, fused to the 
            VP64 transcription activator, in mammalian cells, under a UBC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pUBC_HA_L7Ae-VP64
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..216)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(628..654)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(628..654)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(658..678)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(682..702)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(706..726)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      729..738
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(900..919)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 agctgcaata aacaactgat cagcgggttt aaacgggccc tcatttattc aggtacctca
       61 gttaatcagc atgtccaggt cgaaatcatc aagggcgtca ctgccgagca tgtcgaggtc
      121 aaagtcatca agggcatccg aaccaagcat gtcaaggtca aaatcatcga gggcgtcact
      181 tcccagcata tccagatcaa aatcgtccaa tgcgtcagcc cgtccggaac cggatccacc
      241 ggaacccttc tgcaggccct tgatcttttc caccaggctg cccagttctt tccgcagctc
      301 gccctcgttg atgatggcgg cagaggcaca tggcacctcg atgcccacgg ctctgcccag
      361 gtcgttcttg gacttcacgt agatgtaggg cacgttcttc tcttcgcaca gcaggggcag
      421 atgggccaca atctcggggg ggtccacgtc ctcggcgata tacaccagct tggccaggcc
      481 ccgttccacg gccttggtgg tttcgttggt gcccttcttc actttgccgc tctcgcgcac
      541 tttttccagc aggctcaggg cctcgttctg catgtcctcg ggcacctcga agcgcacgta
      601 catgccgccg gtgccggcac cacttccggc gtagtcgggc acgtcgtagg ggtagccaac
      661 tttccttttc ttcttcggat caactttgcg tttcttcttt gggtccacct ttctcttttt
      721 tttagggtcc atggtggcgg tggccgtacc tccaggtcta acaaaaaagc caaaaacggc
      781 cagaatttag cggacaattt actagtctaa cactgaaaat tacatattga cccaaatgat
      841 tacatttcaa aaggtgccta aaaaacttca caaaacacac tcgccaaccc cgagcgcata
      901 gttcaaaacc ggagcttcag ctacttaaga agataggtac ataaaaccga ccaaagaaac
      961 tgacgcctca cttatccctc ccctcaccag aggtccggcg cctgtcgatt caggagagcc
     1021 taccctaggc ccgaaccctg cgtcctgcga cggagaaaag ccta
//
