LOCUS       sequence-124595-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP1
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..59)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggcggggcgt aaacgccatg ggcatgtagt caaaagcctc cggtcggagg cttttgactt
       61 ggctgaggag tgccgttaat taagtttaaa aatcttcgtt agtttctgct acgccttcgc
      121 tatcatctac agagaaatcc ggcgttgagt tcgggttgct cagcagcaac tcacgtactt
      181 tcttctcgat ctctttcgcg gtttccgggt tatctttcag ccaggcagtc gcattcgctt
      241 taccctgacc gatcttctca cctttgtagc tgtaccacgc gcctgctttc tcgatcagct
      301 tctcttttac gcccaggtca accagttcgc cgtagaagtt gataccttcg ccgtagagga
      361 tctggaattc agcctgttta aacggcgcag cgattttgtt cttcaccact ttcacgcggg
      421 tttcgctacc caccacgttt tcgccctctt tcaccgcgcc gatacgacgg atgtcgagac
      481 gaacagaggc gtagaatttc agcgcgttac caccggtagt ggtttccggg ttaccgaaca
      541 tcacaccaat tttcatacgg atctggttga tgaagatcag cagcgtgttg gactgcttca
      601 ggttacccgc cagcttacgc atcgcctggc tcatcatacg tgccgcaagg cccatgtgag
      661 agtcgccgat ttcgccttcg atttccgctt tcggcgtcag tgccgccacg gagtcaacga
      721 cgataacgtc tactgcgcca gaacgcgcca gggcgtcaca gatttccagt gcctgctcgc
      781 cggtgtccgg ctgggagcac agcaggttat cgatatcgac gcc
//
