LOCUS       sequence-124597-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP2
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(37..68)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gagtggcagg gcggggcgta aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc
       61 ttttgacttg gctgaggagt gccgttaatt aagtttatgc tcgccagagg caacttccgc
      121 ctttcttctg caccagatcg agacgggctt catgagctgc aatctcttca tctgtcgcaa
      181 aaacaacgcg taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt
      241 gctgttgtgt ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca
      301 gataaacttc cgcaaggatc tgggcatcga gtaatgcccc gtgcagcgtt cgtttactgt
      361 tatctatttc gtagcgagca cataacgcat cgaggctgtt gcgcttaccg ggaaacattt
      421 tcctcgccac cgcaaggcta tcggtgacct tacagaaagt attggtcttc ggaatatcgc
      481 gcttaagcaa cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca
      541 ccaactccgc gccgcgaata tagtccatga actcatcggc tacttcggca aacgtgggct
      601 tatcgagcaa aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc
      661 gatcgggttt gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa
      721 cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg
      781 tggttgcggt agcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg
      841 tcagacttga gttgtacgcg tc
//
