LOCUS       sequence-124598-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-B2
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..59)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggcggggcgt aaacgccatg ggcatgtagt caaaagcctc cggtcggagg cttttgactt
       61 ggctgaggag tgccgttaat taagtttagt caaactccag ttccacctgc tccgaaccaa
      121 tgaggccacg gagatcgttt aataaacgat cgctcggaga gacacgccac gtcgcgccaa
      181 aacgcaaccg cgcgcgtgca tccgccctct gatagtagag atgtactgga attgtcccag
      241 agcggtgggg ttccagagac tgacggagtc ggtttaaaag ctggtcatca atttgcctgt
      301 ccgtcagcga gatagcaagc ccgcgagcat atttttcccg ggcttcgtca atatccatca
      361 cttcgcgagc ggtcatttta agcccaccgc tgaagtcatc aaagctgacc tgtccgctga
      421 cgataagtat gcggtctttt tccagcaatt gctggtattt atccagggcg tcagtaaaca
      481 acatcacttc cagccgcccg gaacggtcat ccagcgtgca gataccgata cgattgccgc
      541 gcttggtgac cataacccgc gcggcaacaa cgagccccgc agccgtgatg actttaccac
      601 gttctgtcgg gtgcatgtct ttcagcctta cgcctccgac ataacgctca atctctttta
      661 aatactggtt gatagggtgt ccggtcaggt acaggcctaa cgtttcacgt tccccatcta
      721 ataccacctg ctccggccac ggttggcagc tggcgtagga ttgttcaatt tgttccggct
      781 cttcggccag cacgccgaac atatcggcct gaccgatagc ttccgctt
//
