LOCUS       sequence-124599-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-B4
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(42..73)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcgatgagtg gcagggcggg gcgtaaacgc catgggcatg tagtcaaaag cctccggtcg
       61 gaggcttttg acttggctga ggagtgccgt taattaagtt tagtcaaact ccagttccac
      121 ctgctccgaa ccaatgaggc cacggagatc gtttaataaa cgatcgctcg gagagacacg
      181 ccacgtcgcg ccaaaacgca accgcgcgcg tgcatccgcc ctctgatagt agagatgtac
      241 tggaattgtc ccagagcggt ggggttccag agactgacgg agtcggttta aaagctggtc
      301 atcaatttgc ctgtccgtca gcgagatagc aagcccgcga gcatattttt cccgggcttc
      361 gtcaatatcc atcacttcgc gagcggtcat tttaagccca ccgctgaagt catcaaagct
      421 gacctgtccg ctgacgataa gtatgcggtc tttttccagc aattgctggt atttatccag
      481 ggcgtcagta aacaacatca cttccagccg cccggaacgg tcatccagcg tgcagatacc
      541 gatacgattg ccgcgcttgg tgaccataac ccgcgcggca acaacgagcc ccgcagccgt
      601 gatgacttta ccacgttctg tcgggtgcat gtctttcagc cttacgcctc cgacataacg
      661 ctcaatctct tttaaatact ggttgatagg gtgtccggtc aggtacaggc ctaacgtttc
      721 acgttcccca tctaatacca cctgctccgg ccacggttgg cagctggcgt aggattgttc
      781 aatttgttcc ggctcttcgg ccagcacgcc gaacatatcg gcctgaccga tagcttccgc
      841 tttcgcgtgt tgatctgccg ctt
//
