LOCUS       sequence-124600-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-B5
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(44..75)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctgcgatgag tggcagggcg gggcgtaaac gccatgggca tgtagtcaaa agcctccggt
       61 cggaggcttt tgacttggct gaggagtgcc gttaattaag tttagtcaaa ctccagttcc
      121 acctgctccg aaccaatgag gccacggaga tcgtttaata aacgatcgct cggagagaca
      181 cgccacgtcg cgccaaaacg caaccgcgcg cgtgcatccg ccctctgata gtagagatgt
      241 actggaattg tcccagagcg gtggggttcc agagactgac ggagtcggtt taaaagctgg
      301 tcatcaattt gcctgtccgt cagcgagata gcaagcccgc gagcatattt ttcccgggct
      361 tcgtcaatat ccatcacttc gcgagcggtc attttaagcc caccgctgaa gtcatcaaag
      421 ctgacctgtc cgctgacgat aagtatgcgg tctttttcca gcaattgctg gtatttatcc
      481 agggcgtcag taaacaacat cacttccagc cgcccggaac ggtcatccag cgtgcagata
      541 ccgatacgat tgccgcgctt ggtgaccata acccgcgcgg caacaacgag ccccgcagcc
      601 gtgatgactt taccacgttc tgtcgggtgc atgtctttca gccttacgcc tccgacataa
      661 cgctcaatct cttttaaata ctggttgata gggtgtccgg tcaggtacag gcctaacgtt
      721 tcacgttccc catctaatac cacctgctcc ggccacggtt ggcagctggc gtaggattgt
      781 tcaatttgtt ccggctcttc ggccagcacg ccgaacatat cggcctgacc gatagcttcc
      841 gctttcgcgt gttgatctgc cgctt
//
