LOCUS       sequence-124601-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-C2
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(37..68)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gagtggcagg gcggggcgta aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc
       61 ttttgacttg gctgaggagt gccgttaatt aagtttaaat ggggcggata ctttcttcat
      121 ccagctccgc atcgaagaac cgacgcaggg tctgaatatt attatccgca ataatggact
      181 cgcgcgcctg cgcaagtttt tcttcgtata tcgcctgacg ccatttcagc ggcgtacgca
      241 ccgcgggatt atcatcttca acgatagtca gttcaaccgt tgaacctttt aacatgctca
      301 acgcttcagc cagtttttgc tgtgcaccgc ggttgttcaa atgccgctga gaggagcgca
      361 aatgcagaca tactgcgttg tcgctctcct ctttccaggc atttaacgcc acctgttcga
      421 ccagttttgg tagcgaaagt tggctcacct gtgccgccca cgggtcgcgc tcaatggctt
      481 ctgccgctag cttcgccgcc agttccggcg ttttttcatg ttccagcgct tttttcagcg
      541 ccttcggcgt ggcgaccact tctttttgct gcatcaccgg agtggtcgcc ttccagcgat
      601 acgcttcttt tttggctggc gctttttcca gcgccgatgg caccggacgc gcctgaacgc
      661 gatcggtgac cgaagccagt ctttccagcg cagcgttatt caccggccgc gcgcgggtag
      721 cggctgccgg ttcactcttt tttgctttgg ttgctccctg cacgcgctgc aactgctggc
      781 gcgccgccag cacctggctg gtggtttccg ggagcggtac agtcggtgcc tgctgcggcg
      841 ctgattgcgg ttgcggcggc acctgggttg gcgtcattac tgccgttggc gcgacgggtg
      901 caaaggactg tcgtggcact tctggctcag gcagcggcat acgcgg
//
