LOCUS       sequence-124602-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-C3
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..59)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggcggggcgt aaacgccatg ggcatgtagt caaaagcctc cggtcggagg cttttgactt
       61 ggctgaggag tgccgttaat taagtttaaa tggggcggat actttcttca tccagctccg
      121 catcgaagaa ccgacgcagg gtctgaatat tattatccgc aataatggac tcgcgcgcct
      181 gcgcaagttt ttcttcgtat atcgcctgac gccactccag cggcgtacgc accgcgggat
      241 tatcatcttc aacgatagtc agttcaaccg ttgaaccttt taacatgctc aacgcttcag
      301 ccagtttttg ctgtgcaccg cggttgttca aatgccgctg agaggagcgc aaatgcagac
      361 atactgcgtt gtcgctctcc tctttccagg catttaacgc cacctgttcg accagttttg
      421 gtagcgaaag ttggctcacc tgtgccgccc acgggtcgcg ctcaatggct tctgccgcta
      481 gcttcgccgc cagttccggc gttttttcat gttccagcgc ttttttcagc gccttcggcg
      541 tggcgaccac ttctttttgc tgcatcaccg gagtggtcgc cttccagcga tacgcttctt
      601 ttttggctgg cgctttttcc agcgccgatg gcaccggacg cgcctgaacg cgatcggtga
      661 ccgaagccag tctttccagc gcagcgttat tcaccggccg cgcgcgggta gcggctgccg
      721 gttcactctt ttttgctttg gttgctccct gcacgcgctg caactgctgg cgcgccgcca
      781 gcacctggct ggtggtttcc gggagcggta cagtcggtgc ctgctgcggc gctgattgcg
      841 gttgcggcgg cacctgg
//
