LOCUS       sequence-124604-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-D4
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(39..70)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 atgagtggca gggcggggcg taaacgccat gggcatgtag tcaaaagcct ccggtcggag
       61 gcttttgact tggctgagga gtgccgttaa ttaagtttag tcaaactcca gttccacctg
      121 ctccgaacca atgaggccac ggagatcgtt taataaacga tcgctcggag agacacgcca
      181 cgtcgcgcca aaacgcaacc gcgcgcgtgc atccgccctc tgatagtaga gatgtactgg
      241 aattgtccca gagcggtggg gttccagaga ctgacggagt cggtttaaaa gctggtcatc
      301 aatttgcctg tccgtcagcg agatagcaag cccgcgagca tatttttccc gggcttcgtc
      361 aatatccatc acttcgcgag cggtcatttt aagcccaccg ctgaagtcat caaagctgac
      421 ctgtccgctg acgataagta tgcggtcttt ttccagcaat tgctggtatt tatccagggc
      481 gtcagtaaac aacatcactt ccagccgccc ggaacggtca tccagcgtgc agataccgat
      541 acgattgccg cgcttggtga ccataacccg cgcggcaaca acgagccccg cagccgtgat
      601 gactttacca cgttctgtcg ggtgcatgtc tttcagcctt acgcctccga cataacgctc
      661 aatctctttt aaatactggt tgatagggtg tccggtcagg tacaggccta acgtttcacg
      721 ttccccatct aataccacct gctccggcca cggttggcag ctggcgtagg attgttcaat
      781 ttgttccggc tcttcggcca gcacgccgaa catatcggcc tgaccgatag cttccgcttt
      841 cgcgtgttga tctgccgctt
//
