LOCUS       sequence-124605-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-E
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(39..70)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 atgagtggca gggcggggcg taaacgccat gggcatgtag tcaaaagcct ccggtcggag
       61 gcttttgact tggctgagga gtgccgttaa ttaagtttaa atggggcgga tactttcttc
      121 atccagctcc gcatcgaaga accgacgcag ggtctgaata ttattatccg caataatgga
      181 ctcgcgcgcc tgcgcaagtt tttcttcgta tatcgcctga cgccatttca gcggcgtacg
      241 caccgcggga ttatcatctt caacgatagt cagttcaacc gttgaacctt ttaacatgct
      301 caacgcttca gccagttttt gctgtgcacc gcggttgttc aaatgccgct gagaggagcg
      361 caaatgcaga catactgcgt tgtcgctctc ctctttccag gcatttaacg ccacctgttc
      421 gaccagtttt ggtagcgaaa gttggctcac ctgtgccgcc cacgggtcgc gctcaatggc
      481 ttctgccgct agcttcgccg ccagttccgg cgttttttca tgttccagcg cttttttcag
      541 cgccttcggc gtggcgacca cttctttttg ctgcatcacc ggagtggtcg ccttccagcg
      601 atacgcttct tttttggctg gcgctttttc cagcgccgat ggcaccggac gcgcctgaac
      661 gcgatcggtg accgaagcca gtctttccag cgcagcgtta ttcaccggcc gcgcgcgggt
      721 agcggctgcc ggttcactct tttttgcttt ggttgctccc tgcacgcgct gcaactgctg
      781 gcgcgccgcc agcacctggc tggtggtttc cgggagcggt acagtcggtg cctgctgcgg
      841 cgctgattgc ggttgcggcg gcacctgg
//
