LOCUS       sequence-124609-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-J3
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(38..69)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(713..732)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tgagtggcag ggcggggcgt aaacgccatg ggcatgtagt caaaagcctc cggtcggagg
       61 cttttgactt ggctgaggag tgccgttaat taagtttagg ttggcttttc acgctgctca
      121 gccagctcca gagcggcttt cacgcgagac tgcttcttcg ctttctcacg aatgcttaca
      181 acgtcattcg gactaacctg ataagaagcg atgttaacaa cacgaccgtt taccataatt
      241 gctttatggc taaccagctg acgtgcttct gcacgagtgg caccgaagcc catacggtat
      301 acaacgttgt ccagacgacc ttccagcaga gccaacaggt tttcaccggt gttgcctttc
      361 agacgtgctg cttctttgta gtagttacgg aactgacgct ccagcacacc atagatacgg
      421 cgaacttttt gcttttcacg caactgcaca ccatagtcag acagacgcgg tttacgcgca
      481 ccgtgctggc caggagcttg ttcaatttta cacttggtat cgatcgcgcg aacgccagac
      541 ttaaggaata agtcggtgcc ctcacgacgg ctcagcttga gcttaggacc caaatatctt
      601 gccatagcgg tcatttatgt cagacttgag ttgtacgcgt ccaaaaaaac gggtatggag
      661 aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt atggataaaa
      721 atgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtggact tttctgccgt
      781 gattatagac actt
//
