LOCUS       sequence-12461-e       10496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, 
            Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
  TITLE     A first version of the Caenorhabditis elegans Promoterome.
  JOURNAL   Genome Res. 2004 Oct;14(10B):2169-75.
  PUBMED    15489340
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2004-10"; volume: "14(10B)"; pages: "2169-75"
COMMENT     Sequence Label: pdestDD03
FEATURES             Location/Qualifiers
     source          1..10496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4421..4432
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    6420..6543
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6690..6792
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6793..7452
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6859..6878)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7794..8099
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7978..7997
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8140..8263)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(8308..8326)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8347..8363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8347..8363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8360..8382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8371..8387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8395..8401,8402..8419,8420..8425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8440..8461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8578..8595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8749..9337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8829..8848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9508..10299,10300..10368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10131..10150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10369..10473)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg
      661 cggtggcggc cgctctagag aattttaaca atacttcaaa tttgttaagc cgaacataac
      721 aaaaaatgaa taaaaaacgg aaagtgattg acccgtattt gaagaagaag acaaatgagt
      781 gggggcgaca atgggggaaa aagagacgat gaaagtgaag tggaattaaa tgtcggaaaa
      841 ttttgtggaa aaagaataat gaaatataca tataatatca gtatcttcag cttttcctcc
      901 tgcttcacaa tctcggcggc agatttatgt aaatgatatc tccattcaaa tggtattcaa
      961 agaagaagaa gaagaaggca attgctgata atgagataaa aggaggcttc tcctactgat
     1021 aacaattggt cgggaattca tgattagtgg aagaaaaggg tggtgttttg gatgaagaag
     1081 atgttgggaa tgccaatgat cggatttttg atgcaagcag ttatcttttg caacatgatt
     1141 agtcatttaa atcagggttg gcgacaaaca atttttttcc ggcaaatcgg caaatccgtt
     1201 agttgccgga attggtaact tccggcaaat cgacaaatgg tcggaattgg aatgttcggc
     1261 aaatcggcaa atcggcaatt tgtcggaatt gaaatttccg acaaagcggc aaatcggcag
     1321 tttttcggaa ttgacatttt tggcaaatcg gcaaatcggt tagatgccgg aactgaaaac
     1381 ttccggcaaa gcggcaaatc gctgactttc caatttgatt ttgttgagaa gttttatcta
     1441 aagttaaatt tctagctaat cggcaagtcc gttaattgcc ggaattggaa atttccggca
     1501 aatcggcaaa gcggcacatt gtcggaattg aaatttctag ctaatcggca aatcggttag
     1561 ttgccggaat tggaaacttc cggcaaagcg acaaatcaca gattttccaa atttaatttt
     1621 tatgataatt ttttgtaaaa gggccgaatt atttttgaat aattcaagtt ttttttaatt
     1681 ttatggtttt ttccaacatt caaataaaac atatcaattg agaacattat atttgaaaaa
     1741 tcatttattg ggatttgtat tgaaaagctg aaaaatgaga gttttcggaa tgcattttgt
     1801 gagaaatata gtgaaaaatt aggaaatttt aaatttagcg gtaggaaaat cacatgattt
     1861 ctataaattg cgagaaaaat tattagtttt attccatcgg cgggaaatta tgcatcatac
     1921 gagtagtcgg ccttattgtg cattacgagc ttattctcca caaaatagaa gctatcggag
     1981 cgggtctcgt ttggattatt gatcatgtcg tccactgaaa atatacagtt ttttttttaa
     2041 ttttacaaca aaaaaacata tttttttttt gcaaatttca ttttactttc caattagtca
     2101 gtttttttcc gcgataattt ttaatttcat gggctagttt tggcaagaat tcttctcttc
     2161 gatgcaccat gagcacttaa aaaaaatttg cgaacttttt tatcggttta atgtagtttt
     2221 taacaaaatt tctcaaatgt tcaatacatt tccagaaatt ttgaatttcc cgtcaaataa
     2281 ttttctcaga aaatttgaat tctcgcggct aatttttttc tcagaaaatt tgaattaccc
     2341 gccaaaaact tctcagagaa aactcgaatt tcctccaagt tttcacattt tgaactactg
     2401 tagggcagct ttttgtcaat atttatttta tttttaaaaa cctagaaaat tcgctctatt
     2461 gacaaaatga gctcaaataa tttttcccgc ttcaaactca ctgagttgtt gtgaaagttg
     2521 tggaaattca tagatatgtt cacagttgtt tctcgaattt ggcatacaga atccaaattc
     2581 aaaatcaaag catttcaata aacgatcctt gaaaaagtgc cgttcaatca ttcgaaaatg
     2641 tgtgatcggc acatcgccga ccttgaactc gaccgtcgcg ccgaccgtct ttaatttcag
     2701 aaaattcggc gcaaatcgat atcggacata tcttgccgat tcggcttgcg cctgcagatt
     2761 ctcttccgtc tcattttccg gttttgcaat ttcgaagagc acgtgttccg tgtcgaggtc
     2821 acggatttgg aatttggtga actcaatgtt atagacgttt gccgatgggg agcataggaa
     2881 tcctgaaaat ttgatatgtt tgtccacgtg gagtacacgg cgcgtttatc aatagagcaa
     2941 atattcactt accttgagtg attcctggaa gagctagcac atcatttggt gtaatttgat
     3001 ttttcgcgag aagctccgcc tcggtggtta tagcctgttc ggttaccggt gggggtcggg
     3061 gcatctgaaa aaaaaaagag ttttctgtat tctgccaagg aaacagcaaa ttatgttcgg
     3121 caaaaataga caaaaagtaa ttgagaaaat tgaccggtgc tggcatgcaa tttttgtaca
     3181 tagatgtgaa tttagcctat ttcaatttat tatttttgtg tgaatttttt ttttaatatt
     3241 caaacagtgc acgctttttt gggaaccagg tgttggcggc agttgccgtt cggcaaattg
     3301 caggtttgcc gatttgacga aactttgtat ttccggcaat tttccgtttt ccggaaattt
     3361 ttagagaaat attttataag acggaaacac ttaaaactgt gcctttttga aatttttttt
     3421 cggcaaatca acaatttgcc agaaacttta aattccggca atttgccaat ttgccgattt
     3481 gtcggaaaaa atcatttgcc gccaacccca cgtggtgcca ggctgtccca tcacggtttg
     3541 atatacaaaa aatgcgggaa ttctttgtcc aaaaagatgt gacgtcagca cgttcataaa
     3601 aggggtgtgc ggcaaatctc aaaatttgcc cagctcggca aacggcaaat ttgccgaaca
     3661 cccctggttc ataaccatgt gaaatcagtt gagaactctg cgtctcttct cccgcatttt
     3721 ttgtagatca acgtagatca aaccgaaatg gaacactctg acaccacgtg ccacccctga
     3781 ttgaaccacc gaaaggcaca tatacaaatg ttcaaaaatg agtggcaaaa actagcgaaa
     3841 tttgactgca caaaaaaata atttttaaaa ttttctttta aaatctactg tgttattcgg
     3901 tcattttagc gctataaaag tagattctaa tagtaagtgc aaaattgcaa attaaattta
     3961 tttggagcaa ctctaagtct ctctaaaatc cgcgtttttt gagaaaagtt caaacaattt
     4021 caacgtagaa gaaaagaaag caagcatatc gatatatgaa attggatatc aggagttagt
     4081 atactgcgtg cataaacacg ctctctctct catttctctg cgtcttcctc acctgagacg
     4141 ggaaggttgc cgagccgggt gcgatcgatt gttgttgttg ctctgccttc atatatgctg
     4201 ttgtagctga aaattttggg gattatggga attttggaga gtgctctgga gattagaggt
     4261 ggtgtggctg cggaaaaaat tcggccacca gtattttcta ggccgtgggg gatagggtta
     4321 ttctttaagc aaatttcagg tttccaagtt tttattgtaa attttgagaa ttaaaataaa
     4381 aattataaaa gttctggaaa atatttccgt atgaatttcc tgggaacttc aaattttttt
     4441 gtacaaagtg caaaaatata tttaatttaa attggtttaa aaaattcggc caccagtttt
     4501 ttctatgccg cgaggtatag tgttagtgtg tttttgacaa ctttcaattt tttgagaaaa
     4561 ttttaccaaa aatattccaa gttttcatca taaatttcct gggaacttaa taatattttt
     4621 tgagcacaat ttggtagaaa atattttttc aaattttcaa attttgtaaa attggtgaaa
     4681 tattttgaat tcactttcaa ttttatttat tcaaggaata ttttgaaaaa aaatgtgttt
     4741 ttaattcaca aataattact aaaaataact aaaaaataac actgttttat gggatttaca
     4801 gtttttgttt aaaatcctga aatatttttt ttacttaaac attaataatt tttttatgag
     4861 taaagaaata tgcaattcaa aaatattttt aaaatatttg aacgcatcca attaattgat
     4921 taaatttatg atgtttgcgt ttttttttga gtttttgaaa agatatgttc ccaaaatttt
     4981 tggaaatgtt tgtaaaaatt tttagatttt ttactccgga aacccttttt atggaaaact
     5041 ttcaaacccc tctaatgtac caaaagagca aaaaatcaat ttttgcactg aataaccttt
     5101 taactaacta aaatgaaaaa gaaattccaa caaaaaataa atgagttccg tgtgctcttg
     5161 ctcgcagagc acatccaaaa attaatgggg gtcgtccgta atgattgaca gagatggaaa
     5221 atggcgaaaa agggctgaag aagatgaaaa aagaagacgt ctaggaaaag agaagcgaga
     5281 gagtgtgggg aacgctgagg gcgcagagag aggaacggac aaaatgacag atgatgagca
     5341 aagagaaaga gagagaggga gagagaaaga gagggagaga gggatgaatt tgtgatgaaa
     5401 tagacagtga gagaaaatga gaggaaaaga tggtaattca atagaatctt agagcaattt
     5461 gaagtttgag tcattacttt taaagtgggc atctttaaag gtagaatcat ttaggatttt
     5521 atctaaataa atctataata aaccaatatg actgaatgtc attttggtac actgccaaaa
     5581 ttcaaaaaaa aattggagaa tttgagaaat tttgagcttt gctaccagtt tgaacctctg
     5641 gaacaggggt gggcggcaat tgccttccgg caaatttcgc caaattgccg gtttgccgat
     5701 ttgtcggaaa ttttaatttt cggcaaaaat gcggatttgc tcttttacgg atatcaattt
     5761 gccggaagtt tttagaggga tttagtataa ttcggaaaca cttgaaactg tgcctttttg
     5821 aaattgtttc cccattttct ttagacattt tcatagaatt cgcttacttt tcaaaataga
     5881 tgtaggaaca ttcttaggat gcctacagtt ttgccgatta aaattgaaat tctgaaattt
     5941 ccaaaaaaaa aaattttaaa accacaattt gccattttcg tgtcaaaaac tttcggcaat
     6001 taccgttttt ccggcaaatt cagcaaattg gcaatttgcc ggttccccaa tttgccggaa
     6061 attttcagtt ctggcaattt gccaatggtt tttgaaacat tttacagtaa tatataagga
     6121 tttcatgggt aactatagga ctataggact ataggagtgg tttaaaaaac tgaattttga
     6181 gagacttgac acattttcca aaacattttt tttaatgtta cgtttgactt aaagcaatga
     6241 taagatatgt taagattttg aagaatttag atatgaggtt atagaatact ttgcattagt
     6301 gatgtattta tttgaaagaa cattgatggg gagaattttt caaacagaaa aaaaactata
     6361 aaattctact tcttttactg aagcttggat cctctagagc ggccgccgac tagtgatatc
     6421 aactttgtat agaaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     6481 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     6541 atggcggccg ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     6601 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     6661 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     6721 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     6781 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     6841 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     6901 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     6961 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     7021 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     7081 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     7141 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     7201 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     7261 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     7321 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     7381 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     7441 ggcggggcgt aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg
     7501 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     7561 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     7621 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     7681 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     7741 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
     7801 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     7861 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     7921 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     7981 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     8041 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     8101 tgtcaggctc cgttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     8161 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     8221 atatcatttt acgtttctcg ttcagctttc ttgtacaaag ttgatatctc gacccgggaa
     8281 ttccggaccg gtacccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     8341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8401 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
