LOCUS       sequence-124611-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of the ""TOP1"" construct, targeting the GLuc 
            reporter, in mammalian cells. U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_TOP1
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(462..478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..478)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(475..497)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    486..502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(510..516,517..534,535..540))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    555..576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(693..710)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(944..963)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcttggctt tatatatctt gtggaaagga cgaaacaccg atctagatac gactcactat
       61 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
      121 ttgaaaaagt ggcaccgagt cggtgcttca ttggaattgc gggaaagggg tcaacagccg
      181 ttcagtacca agtctcaggg gaaactttga gatggccttg caaagggtat ggtaataagc
      241 tgacggacat ggtcctaaca cgcagccaag tcctaagtca acagtctgga gcagacgata
      301 tggcgtcgct cctctccacg agagcatatg ggctccgtgg tctccagcag acgatatggc
      361 gtcgctggtc tctgttgata tggatgcagt tcattttttt cttaattaag tctagagtcg
      421 actgtttaaa cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
      481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1021 tccgcctttc tcccttcggg aa
//
