LOCUS       sequence-124612-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of the ""TOP1"" construct, targeting the GLuc 
            reporter, in mammalian cells. U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_TOP1
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(455..474)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(638..655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(638..654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..669)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(863..882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     982..1004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1042..1060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcaggtttaa acagtcgact ctagacttaa ttaagaaaaa aatgaactgc atccatatca
       61 acagagacca gcgacgccat atcgtctgct ggagaccacg gagcccatat gctctcgtgg
      121 agaggagcga cgccatatcg tctgctccag actgttgact taggacttgg ctgcgtgtta
      181 ggaccatgtc cgtcagctta ttaccatacc ctttgcaagg ccatctcaaa gtttcccctg
      241 agacttggta ctgaacggct gttgacccct ttcccgcaat tccaatgaag caccgactcg
      301 gtgccacttt ttcaagttga taacggacta gccttattta aacttgctat gctgtttcca
      361 gcatagctct taaacatagt gagtcgtatc tagatcggtg tttcgtcctt tccacaagat
      421 atataaagcc aagaaatcga aatactttca agttacggta agcatatgat agtccatttt
      481 aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt
      541 ttgtactaat atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt
      601 gtatcgtata tgcaaatatg aaggaatcat gggaaacact ggccgtcgtt ttacaacgtc
      661 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      721 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      781 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      841 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      901 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      961 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     1021 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1081 taataatggt ttcttagacg tcagtg
//
