LOCUS       sequence-12462-e       12102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, 
            Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
  TITLE     A first version of the Caenorhabditis elegans Promoterome.
  JOURNAL   Genome Res. 2004 Oct;14(10B):2169-75.
  PUBMED    15489340
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2004-10"; volume: "14(10B)"; pages: "2169-75"
COMMENT     Sequence Label: pdestMB14
FEATURES             Location/Qualifiers
     source          1..12102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2209..2332
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2357..2362,2363..2380,2381..2387)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2441..3121
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2507..2526)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3441..3746
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3625..3644
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3787..3910)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3958..3986)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4734..4754
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     5697..5713
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5698..5714
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7671..7682)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(11461..11480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11462..11480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11487..11504)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11487..11503)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11496..11518)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11645..12100
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11732..11751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11942..11963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct gggtacatca actttgtata
     2221 gaaaagttga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     2281 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     2341 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     2401 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     2461 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     2521 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2581 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     2641 gaatgctcat ccggaactcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     2701 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     2761 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     2821 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     2881 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     2941 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     3001 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     3061 attacaacag tactgcgatg agtggcaggc ggggcgtaat ctagaggatc cggcttacta
     3121 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     3181 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     3241 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     3301 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     3361 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     3421 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     3481 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     3541 tccccctggc cagtgcacgt ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg
     3601 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     3661 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     3721 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     3781 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     3841 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aactttcttg
     3901 tacaaagttg atgtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt
     3961 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     4021 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     4081 aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta
     4141 aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac
     4201 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     4261 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc
     4321 ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat
     4381 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     4441 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     4501 aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta
     4561 aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     4621 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     4681 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     4741 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaatagcat
     4801 tcgtagaatt ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat
     4861 aataggggcc gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata
     4921 tttaaatttt cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     4981 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaaa
     5041 cttcttctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     5101 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     5161 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     5221 ttttttactt ctgataaatt ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     5281 ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc
     5341 tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc
     5401 aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg gtttccccta
     5461 ttgtttgtca agagtttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     5521 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     5581 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     5641 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cccccctcga
     5701 ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt tttttctgtt
     5761 tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc aaagtattct
     5821 ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct ttaagtcaaa
     5881 cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt cagtttttta
     5941 aaccactcct atagtcctat agtcctatag ttacccatga aatccttata tattactgta
     6001 aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca aattggggaa
     6061 ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga aagtttttga
     6121 cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca gaatttcaat
     6181 tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt gaaaagtaag
     6241 cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg cacagtttca
     6301 agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat atccgtaaaa
     6361 gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa accggcaatt
     6421 tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt caaactggta
     6481 gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca gtgtaccaaa
     6541 atgacattca gtcatattgg tttattatag atttatttag ataaaatcct aaatgattct
     6601 acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct ctaagattct
     6661 attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca caaattcatc
     6721 cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc atctgtcatt
     6781 ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt ctcttttcct
     6841 agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc tctgtcaatc
     6901 attacggacg acccccatta atttttggat gtgctctgcg agcaagagca cacggaactc
     6961 atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta ttcagtgcaa
     7021 aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc ataaaaaggg
     7081 tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt gggaacatat
     7141 cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta attggatgcg
     7201 ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa aaaattatta
     7261 atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc ccataaaaca
     7321 gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat tttttttcaa
     7381 aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca attttacaaa
     7441 atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat tattaagttc
     7501 ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca aaaaattgaa
     7561 agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg tggccgaatt
     7621 ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat ttgaagttcc
     7681 caggaaattc atacggaaat attttccaga acttttataa tttttatttt aattctcaaa
     7741 atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta tcccccacgg
     7801 cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa tctccagagc
     7861 actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat atgaaggcag
     7921 agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag gtgaggaaga
     7981 cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc ctgatatcca
     8041 atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt tgaacttttc
     8101 tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta atttgcaatt
     8161 ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa cacagtagat
     8221 tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta gtttttgcca
     8281 ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt ggcacgtggt
     8341 gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg ggagaagaga
     8401 cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc aaatttgccg
     8461 tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa cgtgctgacg
     8521 tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt gatgggacag
     8581 cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc aaattggcaa
     8641 attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa tttcaaaaag
     8701 gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc ggaaaacgga
     8761 aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc cgaacggcaa
     8821 ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa aaaaaaaatt
     8881 cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa aattgcatgc
     8941 cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat aatttgctgt
     9001 ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc ccaccggtaa
     9061 ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt acaccaaatg
     9121 atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta ttgataaacg
     9181 cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg ctccccatcg
     9241 gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga cacggaacac
     9301 gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca ggcgcaagcc
     9361 gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt aaagacggtc
     9421 ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg aatgattgaa
     9481 cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg attctgtatg
     9541 ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc acaacaactc
     9601 agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc gaattttcta
     9661 ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt caaaatgtga
     9721 aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt caaattttct
     9781 gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga cgggaaattc
     9841 aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac attaaaccga
     9901 taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa gaattcttgc
     9961 caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat tggaaagtaa
    10021 aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa aaaactgtat
    10081 attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata gcttctattt
    10141 tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg cataatttcc
    10201 cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg tgattttcct
    10261 accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg cattccgaaa
    10321 actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa atataatgtt
    10381 ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa aaaacttgaa
    10441 ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa atttggaaaa
    10501 tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga tttgccgatt
    10561 agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa atttccaatt
    10621 ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa acttctcaac
    10681 aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag ttccggcatc
    10741 taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat ttgccgcttt
    10801 gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca ttccaattcc
    10861 gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt tgccgatttg
    10921 ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt tgcaaaagat
    10981 aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca tccaaaacac
    11041 cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag gagaagcctc
    11101 cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac catttgaatg
    11161 gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa agctgaagat
    11221 actgatatta tatgtatatt tcattattct ttttccacaa aattttccga catttaattc
    11281 cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt gtcttcttct
    11341 tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt cggcttaaca
    11401 aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggag ctccaattcg
    11461 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    11521 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11581 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    11641 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    11701 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    11761 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    11821 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    11881 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    11941 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    12001 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    12061 tttaacgcga attttaacaa aatattaacg cttacaattt ag
//
