LOCUS       sequence-124625-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of GFP tag at Atg16 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD116
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      45..232
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(100..124)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        289..504
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 ggcatggatg aactatacaa atagggcgcg ccacttctaa ataagcgaat ttcttatgat
       61 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
      121 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
      181 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
      241 ctagggataa cagggtaata tagatctgag agtgcaccat accacctttt caattcatca
      301 tttttttttt attctttttt ttgatttcgg tttccttgaa atttttttga ttcggtaatc
      361 tccgaacaga aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata
      421 tgtagtgttg aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa
      481 acctgcagga aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact
      541 catcctagtc ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg
      601 tgtgcttcat tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt
      661 cccaaaattt gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc
      721 acagttaagc cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga
      781 aaatttgctg acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata
      841 gcagaatggg cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt
      901 ttgaagcagg cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa
      961 ttgtcatgca agggctccct atctactgga gaatatacta agggtactgt tgacattgcg
     1021 aagagcgaca a
//
