LOCUS       sequence-124627-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of GFP tag at Atg16 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD116
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..783)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(113..133)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     728..756
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttttataac ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag
       61 tggcgcgccc tatttgtata gttcatccat gccatgtgta atcccagcag ctgttacaaa
      121 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt
      181 ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg
      241 ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttaac
      301 tttgattcca ttcttttgtt tgtctgccat gatgtataca ttgtgtgagt tatagttgta
      361 ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt ttaactcgat
      421 tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt agttcccgtc
      481 atctttgaaa aatatagttc tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa
      541 gtcatgccgt ttcatatgat ctgggtatct tgaaaagcat tgaacaccat aagtgaaagt
      601 agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt
      661 aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt tgtgcccatt
      721 aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt
      781 actgttaatt aaccc
//
