LOCUS       sequence-124633-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression in mammalian cells of an ""INT"" 
            construct_bearing three MS2 Stem-loops, targeting the GLuc reporter.
            U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_INT[3xMS2_SL]
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(284..303)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(467..484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(467..483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(476..498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(692..711)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     811..833
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(871..889)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtttaaaca gtcgactcta gacttaatta agaaaaaaag caccgactcg gtgccacttt
       61 ttcaagttga taacggacta gccttattta aacttgctat gctgcgaata cgagggtacg
      121 ctgatgctgt accagacaga ccctgatggt gtctgagacg taccctgatg gtgtacgctc
      181 gtattcgcag catagctctt aaacatagtg agtcgtatct agatcggtgt ttcgtccttt
      241 ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata
      301 gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg
      361 tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct
      421 aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaacactg gccgtcgttt
      481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      601 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
      661 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      721 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      781 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      841 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
      901 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgc
//
