LOCUS       sequence-124639-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression in mammalian cells of an ""INT"" 
            construct_bearing the ""Bunch of Baby Spinach"" (or ""BoBS"") 
            construct, targeting the GLuc reporter. U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_INT[BoBS]
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(356..375)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(539..556)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(539..555)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..570)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(764..783)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     883..905
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(943..961)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctgcaggtt taaacagtcg actctagact taattaagaa aaaaagcacc gactcggtgc
       61 cactttttca agttgataac ggactagcct tatttaaact tgctatgctg cgaatacgag
      121 ctcacactct actcaacgga cgcctcacac tctactcaac gaccgactca cactctactc
      181 aacaatggtg tttccaccat tggacccgtc ctttcggtcg gacccgtcct tgcgtccgga
      241 cccgtccttc tcgtattcgc agcatagctc ttaaacatag tgagtcgtat ctagatcggt
      301 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
      361 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
      421 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattcc
      481 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaacac
      541 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      601 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      661 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      721 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
      781 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      841 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      901 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
      961 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1021 gaaatgtgcg cg
//
