LOCUS       sequence-124643-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  General Purpose cloning vector for ""INT""-like constructs under 
            U6-promoter expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(Gluc)_INT(GenPurpClon)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(256..272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(256..272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(269..291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    280..296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(304..310,311..328,329..334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    349..370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(487..504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(738..757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgatctag atacgactca
       61 ctatgtttaa gagctatgct gcgaatacga gaagtcttct tttttgaaga caatcgtatt
      121 cgcagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      181 tcggtgcttt ttttcttaat taagtctaga gtcgactgtt taaacctgca ggcatgcaag
      241 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      961 tc
//
