LOCUS       sequence-124644-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  General Purpose cloning vector for ""INT""-like constructs under 
            U6-promoter expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(Gluc)_INT(GenPurpClon)
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(245..264)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(428..445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(428..444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(437..459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(653..672)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     772..794
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(832..850)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cagggtttaa acagtcgact ctagacttaa ttaagaaaaa aagcaccgac tcggtgccac
       61 tttttcaagt tgataacgga ctagccttat ttaaacttgc tatgctgcga atacgattgt
      121 cttcaaaaaa gaagacttct cgtattcgca gcatagctct taaacatagt gagtcgtatc
      181 tagatcggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca
      241 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc
      301 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa
      361 attaattcca attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat
      421 gggaaacact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      481 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      541 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
      601 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      661 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      721 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      781 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      841 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttat
//
