LOCUS       sequence-124646-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of the ""TOP2"" construct, targeting the GLuc 
            reporter, in mammalian cells. CMV/3' Box expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/3' Box_(GLuc)_TOP2
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(74..149)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(539..742)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(568..588)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gccaccagac acaaaccaaa caaaaaaaga tatgaccctt agcgtacagt ctacttttga
       61 aactccagaa agtgcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
      121 ttttaacttg ctatttctag ctctaaaaca tagtgagtcg tatctagatc gggtttagag
      181 gagggatgtt gaagatgaac tgcatccata tcaacagaaa aagcagcgac gccatatcgt
      241 ctgctgcaaa aaggagcgag cccatatgct ctgctccaaa aaccagcgac gccatatcgt
      301 ctgctggaaa aaccacggag cccatatgct ctgctggaaa aaggagcgac gccatatcgt
      361 ctgctccaaa aactgttgac ttaggacttg gctgcgtgtt aggaccatgt ccgtcagctt
      421 attaccatac cctttgcaag gccatctcaa agtttcccct gagacttggt actgaacggc
      481 tgttgacccc tttcccgcaa ttccgccagt aagcagtggg ttctctagtt agccagagag
      541 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      601 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      661 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      721 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      781 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      841 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      901 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      961 tgggaacata cgtcatt
//
