LOCUS       sequence-124650-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For E17TAG GFP reporter expression in non-zeocin resistant strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, 
            Isaacs FJ, Rinehart J
  TITLE     A flexible codon in genomically recoded Escherichia coli permits 
            programmable protein phosphorylation.
  JOURNAL   Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
  PUBMED    26350500
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-9"; volume: "6:8130. doi"; pages: " 
            10.1038/ncomms9130"
COMMENT     Sequence Label: E17TAG GFP zeo resistance
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             623..634
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cagattgtgt cgacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag
       61 tattttgttg ataatggtct gctagttgaa cggatccatc ttcaatgttg tggcgaattt
      121 tgaagttagc tttgattcca ttcttttgtt tgtctgccgt gatgtataca ttgtgtgagt
      181 tatagttgta ttcgagtttg tgtccgagaa tgtttccatc ttctttaaaa tcaatacctt
      241 ttaactcgat acgattaaca agggtatcac cttcaaactt gacttcagca cgcgtcttgt
      301 agttcccgtc atctttgaaa gatatagtgc gttcctgtac ataaccttcg ggcatggcac
      361 tcttgaaaaa gtcatgccgt ttcatgtgat ccggataacg ggaaaagcat tgaacaccat
      421 aagagaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa
      481 atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt
      541 tgtgcccatt aacatcacca tctaactaaa caagaattgg gacaactcca gtgaaaagtt
      601 cttctccttt actcatggta cctttctcct ctttaatgaa ttc
//
