LOCUS       sequence-124651-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. 
            U1Pro/SmBox/3' Box expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU1/Sm/3' Box_(GLuc)_INT
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(801..817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(801..817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(814..836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    825..841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(849..855,856..873,874..879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    894..915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1032..1049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggagagaaca gacgcagggg cgggagggaa aaagggagag gcagacgtca cttccccttg
       61 gcggctctgg cagcagattg gtcggttgag tggcagaaag gcagacgggg actgggcaag
      121 gcactgtcgg tgacatcacg gacagggcga cttctatgta gatgaggcag cgcagaggct
      181 gctgcttcgc cacttgctgc ttcaccacga aggagttccc gtgccctggg agcgggttca
      241 ggaccgctga tcggaagtga gaatcccagc tgtgtgtcag ggctggaaag ggctcgggag
      301 tgcgcggggc aagtgaccgt gtgtgtaaag agtgaggcgt atgaggctgt gtcggggcag
      361 aggcccaaga tctcagatct agatacgact cactatgttt aagagctatg ctgcgaatac
      421 gagggagcag acgatatggc gtcgctcctc tccacgagag catatgggct ccgtggtctc
      481 cagcagacga tatggcgtcg ctggctcgta ttcgcagcat agcaagttta aataaggcta
      541 gtccgttatc aacttgaaaa agtggcaccg agtcggtgcc agcaagttca gagaaatctg
      601 aacttgctgg atttttggag cagggagatg gaataggagc ttgctccgtc cactccacgc
      661 atcgacctgg tattgcagta cctccaggaa cggtgcaccc actttctgga gtttcaaaag
      721 tagactgtac gctaagggtc atatcttttt ttgtttggtt tgtgtcttgg ttggcgtctt
      781 aaaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      841 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      901 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1021 tcttccgctt cctcgctcac tgactcgctg
//
