LOCUS       sequence-124652-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. 
            U1Pro/SmBox/3' Box expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU1/Sm/3' Box_(GLuc)_INT
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(804..821)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(804..820)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..835)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1029..1048)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttagagcca ccagaacaaa ccaaacaaaa aaagatatga cccttagcgt acagtctact
       61 tttgaaactc cagaaagtgg gtgcaccgtt cctggaggta ctgcaatacc aggtcgatgc
      121 gtggagtgga cggagcaagc tcctattcca tctccctgct ccaaaaatcc agcaagttca
      181 gatttctctg aacttgctgg caccgactcg gtgccacttt ttcaagttga taacggacta
      241 gccttattta aacttgctat gctgcgaata cgagccagcg acgccatatc gtctgctgga
      301 gaccacggag cccatatgct ctcgtggaga ggagcgacgc catatcgtct gctccctcgt
      361 attcgcagca tagctcttaa acatagtgag tcgtatctag atctgagatc ttgggcctct
      421 gccccgacac agcctcatac gcctcactct ttacacacac ggtcacttgc cccgcgcact
      481 cccgagccct ttccagccct gacacacagc tgggattctc acttccgatc agcggtcctg
      541 aacccgctcc cagggcacgg gaactccttc gtggtgaagc agcaagtggc gaagcagcag
      601 cctctgcgct gcctcatcta catagaagtc gccctgtccg tgatgtcacc gacagtgcct
      661 tgcccagtcc ccgtctgcct ttctgccact caaccgacca atctgctgcc agagccgcca
      721 aggggaagtg acgtctgcct ctcccttttt ccctcccgcc cctgcgtctg ttctctccca
      781 aagaagctgg tccttaggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      841 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      901 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      961 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     1021 actctcagta caatctgctc tgatgccgca tagctcgtga tacgcctatt tttataggtt
     1081 aatgtc
//
