LOCUS       sequence-124654-        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of the ""TOP1"" construct, targeting the GLuc 
            reporter, in mammalian cells. CMV/MASC expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/MASC_(GLuc)_TOP1
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..46)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     81..100
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_RNA        complement(644..719)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(775..978)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(804..824)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtttaaacag tcgaataaga tacattgatg agtttggaca aaccacaact agaatgcagt
       61 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      121 gctgcaataa acaaaagcaa agacaccgca gggacttgaa ccccgtcctg gaaaccagga
      181 gtgccagcca ccagcgtctt ttgctttttt ttttttaaag ctagggaaag gccaaaaagc
      241 aaaacctgag aaaacaaaag gttgttttct caggaaaaga aaaaccttta caaccctact
      301 gacgaatcga gaactgcatc catatcaaca gaaaaagcag cgacgccata tcgtctgctg
      361 caaaaaggag cgagcccata tgctctgctc caaaaaccag cgacgccata tcgtctgctg
      421 gaaaaaccac ggagcccata tgctctgctg gaaaaaggag cgacgccata tcgtctgctc
      481 caaaaactgt tgacttagga cttggctgcg tgttaggacc atgtccgtca gcttattacc
      541 ataccctttg caaggccatc tcaaagtttc ccctgagact tggtactgaa cggctgttga
      601 cccctttccc gcaattccgg gtttagagga gggatgttga agagcaccga ctcggtgcca
      661 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaaca
      721 tagtgagtcg tatctagatc gccagtaagc agtgggttct ctagttagcc agagagctct
      781 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      841 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      901 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
      961 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     1021 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     1081 gacgtcaata gggggcgtac ttggcatatg atacact
//
