LOCUS       sequence-124655-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of the ""TOP1"" construct, targeting the GLuc 
            reporter, in mammalian cells. CMV/MASC expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/MASC_(GLuc)_TOP1
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        64..139
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(683..702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     737..756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(813..829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(826..848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    837..853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(861..867,868..885,886..891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcgatctaga tacgactcac
       61 tatgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      121 agtggcaccg agtcggtgct cttcaacatc cctcctctaa acccggaatt gcgggaaagg
      181 ggtcaacagc cgttcagtac caagtctcag gggaaacttt gagatggcct tgcaaagggt
      241 atggtaataa gctgacggac atggtcctaa cacgcagcca agtcctaagt caacagtttt
      301 tggagcagac gatatggcgt cgctcctttt tccagcagag catatgggct ccgtggtttt
      361 tccagcagac gatatggcgt cgctggtttt tggagcagag catatgggct cgctcctttt
      421 tgcagcagac gatatggcgt cgctgctttt tctgttgata tggatgcagt tctcgattcg
      481 tcagtagggt tgtaaaggtt tttcttttcc tgagaaaaca accttttgtt ttctcaggtt
      541 ttgctttttg gcctttccct agctttaaaa aaaaaaaagc aaaagacgct ggtggctggc
      601 actcctggtt tccaggacgg ggttcaagtc cctgcggtgt ctttgctttt gtttattgca
      661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttattc gactgtttaa
      781 acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      841 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      901 gcctaatgag tgagcta
//
