LOCUS       sequence-124657-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ 
            MASC expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/MASC_(GLuc)_INT
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(505..524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     559..578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(635..651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(635..651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(648..670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    659..675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(683..689,690..707,708..713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    728..749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(866..883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcgatctag atacgactca
       61 ctatgtttta gagctacgaa tacgagggag cagacgatat ggcgtcgctc ctctccacga
      121 gagcatatgg gctccgtggt ctgcagcaga cgatatggcg tcgctgctct cgtagatgcc
      181 atatggggca ctacgtctcc agcagacgat atggcgtcgc tggctcgtat tcgtagcaag
      241 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctctcgat
      301 tcgtcagtag ggttgtaaag gtttttcttt tcctgagaaa acaacctttt gttttctcag
      361 gttttgcttt ttggcctttc cctagcttta aaaaaaaaaa gcaaaagacg ctggtggctg
      421 gcactcctgg tttccaggac ggggttcaag tccctgcggt gtctttgctt ttgtttattg
      481 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      541 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat tcgactgttt
      601 aaacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1081 gctcaagt
//
