LOCUS       sequence-124658-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ 
            MASC expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/MASC_(GLuc)_INT
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..54)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     89..108
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(604..807)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(633..653)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        808..1111
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcctgcaggt ttaaacagtc gaataagata cattgatgag tttggacaaa ccacaactag
       61 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      121 cattataagc tgcaataaac aaaagcaaag acaccgcagg gacttgaacc ccgtcctgga
      181 aaccaggagt gccagccacc agcgtctttt gctttttttt tttaaagcta gggaaaggcc
      241 aaaaagcaaa acctgagaaa acaaaaggtt gttttctcag gaaaagaaaa acctttacaa
      301 ccctactgac gaatcgagag caccgactcg gtgccacttt ttcaagttga taacggacta
      361 gccttatttt aacttgctac gaatacgagc cagcgacgcc atatcgtctg ctggagacgt
      421 agtgccccat atggcatcta cgagagcagc gacgccatat cgtctgctgc agaccacgga
      481 gcccatatgc tctcgtggag aggagcgacg ccatatcgtc tgctccctcg tattcgtagc
      541 tctaaaacat agtgagtcgt atctagatcg ccagtaagca gtgggttctc tagttagcca
      601 gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
      661 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
      721 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
      781 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
      841 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
      901 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
      961 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     1021 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     1081 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     1141 tgaccc
//
