LOCUS       sequence-124664-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of humanized MS2 (V75E/A81G), fused to mCherry 
            in mammalian cells, under a UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pUBC_V5_MS2-mCherry
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             153..173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             180..221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
       61 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
      121 gacctggagg taccgccacc gccaccatgg ctccaaagaa gaagcgtaag gtaggcagcg
      181 gcaagcccat ccccaacccc ctgctgggcc tggacagcac cggcagcgct agcaatttca
      241 ctcaatttgt gctcgtggat aacggaggaa cgggcgacgt gaccgtggca ccaagcaact
      301 ttgctaatgg agtggccgag tggattagct caaatagcag gtcccaggct tacaaggtca
      361 cctgtagtgt ccggcaatca tccgcccaga atcggaaata tactattaag gtagaagtgc
      421 cgaaagtggc aactcaaacc gtggggggcg aggaattgcc tgtggccggc tggagaagct
      481 acctcaacat ggaactcacc attccaatct tcgccacaaa cagcgactgt gagctcatcg
      541 tcaaagccat gcagggtctg ctgaaggacg gcaatcctat tccatctgca atcgcggcca
      601 attccggcat ttatggatcc aagggctcgg gctcgacctc gggctccgga atggtgagca
      661 agggcgagga ggacaacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      721 agggctccgt gaacggccgc gagttcgaga tcgagggcga gggcgagggc c
//
