LOCUS       sequence-124665-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of humanized MS2 (V75E/A81G), fused to mCherry 
            in mammalian cells, under a UBC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pUBC_V5_MS2-mCherry
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..737)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(107..130)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(305..324)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     574..592
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gctgcaataa acaagtcgac ttatcattac ttgtacagct cgtccatgcc gccggtggag
       61 tggcggccct cggcgcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
      121 atgtccagct tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
      181 taggtggtct tgacctcggc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
      241 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
      301 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
      361 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
      421 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
      481 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
      541 aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg
      601 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgcg gccgttcacg
      661 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttgtcctcc
      721 tcgcccttgc tcaccattcc ggagcccgag gtcgagcccg agcccttgga tccataaatg
      781 ccggaattgg ccgcgattgc agatggaata ggattgccgt ccttcagcag accctgcatg
      841 gctttgacga tgagctcaca gtcgctgttt gtggcgaaga ttggaatggt gagttccatg
      901 ttgaggtagc ttctccagcc ggccacaggc aattcctcgc cccccacggt ttgagttgcc
      961 actttcggca cttctacctt aatagtatat ttccgattct gggcggatga ttgccggaca
     1021 ctacaggtga ccttgtaagc ctgggacct
//
