LOCUS       sequence-124673-        1139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression in mammalian cells of an ""INT"" 
            construct_bearing three kink-turns, targeting the GLuc reporter. U6 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_INT[3xK-T]
FEATURES             Location/Qualifiers
     source          1..1139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(327..346)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(510..527)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(510..526)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(519..541)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(735..754)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     854..876
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(914..932)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1000..1104
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcctgcaggt ttaaacagtc gactctagac ttaattaaga aaaaaagcac cgactcggtg
       61 ccactttttc aagttgataa cggactagcc ttatttaaac ttgctatgct gcgaatacga
      121 gagactgcct caggctttcg ccatcacggg cagagactgg ctcaggcttt cgccatcacg
      181 gccagagagc tcctcaggct ttcgccatca cgggagcaga ctcgtattcg cagcatagct
      241 cttaaacata gtgagtcgta tctagatcgg tgtttcgtcc tttccacaag atatataaag
      301 ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata
      361 attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta
      421 atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc ttgtatcgta
      481 tatgcaaata tgaaggaatc atgggaaaca ctggccgtcg ttttacaacg tcgtgactgg
      541 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      601 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      661 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      721 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
      781 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      841 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      901 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      961 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1021 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1081 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattcc
//
