LOCUS       sequence-124677-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of an ""INT"" construct_bearing three PP7 
            Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' 
            Box expression backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pCMV/3' Box_(GLuc)_INT
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(363..566)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(392..412)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        567..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gccaaccaag acacaaacca aacaaaaaaa gatatgaccc ttagcgtaca gtctactttt
       61 gaaactccag aaagtaaagc accgactcgg tgccactttt tcaagttgat aacggactag
      121 ccttatttta acttgctacg aatacgagcc agcgacgcca tatcgtctgc tggagacgta
      181 gtgccccata tggcatctac gagagcagcg acgccatatc gtctgctgca gaccacggag
      241 cccatatgct ctcgtggaga ggagcgacgc catatcgtct gctccctcgt attcgtagct
      301 ctaaaacata gtgagtcgta tctagatcgc cagtaagcag tgggttctct agttagccag
      361 agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      421 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      481 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
      541 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
      601 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
      661 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
      721 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
      781 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
      841 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
      901 gaccccgtaa ttgattacta ttaat
//
