LOCUS       sequence-124684-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD214
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..786)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(73..786)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tttttataac ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag
       61 tggcgcgcct tatttatata attcatccat accatgggta ataccagcag cagtaacaaa
      121 ttctaacaag accatgtggt ctctcttttc gtttggatct ttggataagg cagattgagt
      181 ggataagtaa tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg
      241 ataatggtca gctaattgaa cagaaccatc ttcaatgttg tgtctaattt tgaagttaac
      301 tttgatacca ttcttttgtt tgtcagccat gatgtaaaca ttgtgagagt tatagttgta
      361 ttccaatttg tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat
      421 tctattaact aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc
      481 atctttgaaa aaaatagttc tttcttgaac ataaccttct ggcatggcag acttgaaaaa
      541 gtcatgttgt ttcatatgat ctgggtatct agaaaaacat tgaacaccat aagttaaagt
      601 agtgactaag gttggccatg gaactggcaa tttaccagta gtacaaataa attttaaggt
      661 caatttaccg taagtagcat caccttcacc ttcaccggag acagaaaatt tgtgaccatt
      721 aacatcacca tctaattcaa ccaaaattgg gacaacacca gtgaataatt cttcaccttt
      781 agacatgaat ggcttctcta gatcacgcgt tggaactgga attttgtaca attcatcaat
      841 accatgggta ataccagcag cagtaacaaa ttctaacaag accatgtggt ctctcttttc
      901 gtttggatct ttggataagg cagattgagt ggataagtaa tggttgtctg gtaacaagac
      961 tggaccatca ccaattggag tattttgttg ataatggtca gctaattgaa cagaaccatc
     1021 ttcaatgttg tgtctaattt tgaagttaac tttgatacca ttcttttgtt tgtcagccat
     1081 gatgtaaaca ttgtgagagt tatagttgta ttccaatttg tgac
//
