LOCUS       sequence-124687-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of GFP tag at Atg31 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD131
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(938..957)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(939..957)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(964..981)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(964..980)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttgtgaccat taacatcacc atctaattca accaaaattg ggacaacacc agtgaataat
       61 tcttcacctt tagacatgtt aattaaacca gcaccgtcac cgatccgtcg acctgcagcg
      121 tacgaagcta cggaattgga gagcatttgt aattgttcat tttgtgtgac gtagagttta
      181 attaagtcat tcaaactcaa atcgcgaaga aaaggagaca gatcgcaaaa ttgtgaaatt
      241 acatcgagct caatgtccga gtccactctg aatctatcac tgtcaacgct aagttcttcc
      301 tcttcatcat tagatttatc atcaccatgt gatgataatg ttctcatttg gttttcctcc
      361 aagctgagac tatttgtcct atgtgacaaa agctcatatt ggtcgctgat tagtgtgata
      421 tcctcaagag atcccgattc atccaattgg acaattatga cattttcaag ttcatctccg
      481 accttatcta ttcctgctgt caaagcagca tcacttgaat ctactagttc atcattgttg
      541 tcctcaaata tgtacttaat gttagtggga aacattgcat aatcacttcc gtccgtgctc
      601 tttgattcat tattgtatgc tggttgatca tcgttgctgg gacccttgtt atttttgata
      661 ttctcttcta atctgtactt gacattttta tcataaacag taactgtaac attcattcct
      721 gttcgcagat ttcagcaatg tttaccggct taataagaaa atgggaatgc gacgtttatc
      781 ttggatattg accaccctta tattatggat atgtacttca aattattatc ttttacgata
      841 aaaaagattc tgatccaacc gaggttcgaa ctcgggatct tcgccgtgta aaggcgacgt
      901 cttgaaccac tggaccccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa
      961 ttcactggcc gtcgttttac aacgtcgtga ctgg
//
