LOCUS       sequence-124696-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-IN S3 variant 5' UTR sequence controlling expression of 
            superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol.,
            2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAINS3
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..236
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(439..466)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(482..553)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(629..649)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
       61 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      121 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      181 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      241 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      301 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
      361 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactg caggcaaaac
      421 ccgtacccta ggtataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt
      481 agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
      541 ttcgttttat ttgatgcctg gagatcctta ctcgagtttg gatccttatt tgtagagctc
      601 atccatgcca tgtgtaatcc cagcagcagt tacaaactca agaaggacca tgtggtcacg
      661 cttttcgttg ggatctttcg aaaggacaga ttgtgtcgac aggtaatggt tgtctggtaa
      721 aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgga
      781 accatcttca acgttgtggc gaattttgaa gttagctttg attccattct tttgtttgtc
      841 tgccgtgatg tatacattgt gtgagttaaa gttgtactcg agtttgtgtc caagaa
//
