LOCUS       sequence-124700-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of
            superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol.,
            2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAINS4-C24AA25C
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        182..216
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(289..317)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
       61 cacctgaagt cagccccata cgatataagt tgtaattctc atgtttgaca gcttatcatc
      121 gataagcttc cgatggcgcg ccgagaggct ttacacttta tgcttccggc tgaattcgaa
      181 tttgacagct agctcagtcc taggtataat actagtgcca aaaatcaata aggagacaaa
      241 cagatgtgcg aactcgatgc tagcaaagga gaagaacttt tcactggagt tgtcccaatt
      301 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa
      361 ggtgatgcta caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct
      421 gttccgtggc caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat
      481 ccggatcaca tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      541 gaacgcacta tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt
      601 gaaggtgata cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga
      661 aacattcttg gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca
      721 gacaaacaaa agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt
      781 tccgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      841 ttaccagaca accattacct gtcgacacaa tctgtccttt cgaaagatcc ca
//
