LOCUS       sequence-124703-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-IN S4 A25C variant 5' UTR sequence controlling expression of 
            superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol.,
            2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAINS4-A25C
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        181..215
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(288..316)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     911..931
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
       61 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
      121 ataagcttcc gatggcgcgc cgagaggctt tacactttat gcttccggct gaattcgaat
      181 ttgacagcta gctcagtcct aggtataata ctagtgccaa aaatcaataa ggagacaacc
      241 agatgtgcga actcgatgct agcaaaggag aagaactttt cactggagtt gtcccaattc
      301 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag
      361 gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg
      421 ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc
      481 cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      541 aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg
      601 aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa
      661 acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac atcacggcag
      721 acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt
      781 ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      841 taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc
      901 gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg
      961 agctct
//
