LOCUS       sequence-124704-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-IN S4 A25C variant 5' UTR sequence controlling expression of 
            superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol.,
            2012).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Watters KE, Abbott TR, Lucks JB
  TITLE     Simultaneous characterization of cellular RNA structure and function
            with in-cell SHAPE-Seq.
  JOURNAL   Nucleic Acids Res. 2015 Sep 8. pii: gkv879.
  PUBMED    26350218
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Sep 8. pii: gkv879."
COMMENT     Sequence Label: pJBL-RNAINS4-A25C
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..238
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(441..468)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(484..555)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(631..651)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
       61 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
      121 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
      181 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
      241 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
      301 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
      361 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tgcaggcaaa
      421 acccgtaccc taggtataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta
      481 gtagagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
      541 ctttcgtttt atttgatgcc tggagatcct tactcgagtt tggatcctta tttgtagagc
      601 tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac catgtggtca
      661 cgcttttcgt tgggatcttt cgaaaggaca gattgtgtcg acaggtaatg gttgtctggt
      721 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
      781 gaaccatctt caacgttgtg gcgaattttg aagttagctt tgattccatt cttttgtttg
      841 tctgccgtga tgtatacatt gtgtgagtta aagttgtact cgagtttgtg tccaagaatg
      901 tttccatctt ctttaaaatc aatacccttt aactcgatac gattaacaag ggtatcacct
      961 tcaaacttga cttcagcacg cgtcttgtag gtcccgtcat cttt
//
