LOCUS       sequence-124718-        2536 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  artificial transcription template ""terminator insertion"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J,
            Gelles J
  TITLE     Bacterial RNA polymerase can retain sigma70 throughout 
            transcription.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899.
  PUBMED    26733675
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 5. pii: 201513899."
COMMENT     Sequence Label: pTH09
FEATURES             Location/Qualifiers
     source          1..2536
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..27
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2450..2467)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2450..2466)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2459..2481)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattccag acaacatttt
       61 gaatgcggtc acacgttagc agcatgattg ccacggatgg caacatatta acggcatgat
      121 attgacttat tgaataaaat tgggtaaatt tgactcaacg atgggttaat tcgctactga
      181 gctaataaca ggcctgctgg taatcgcagg cctttttatt tggattcgga tccagacacc
      241 acagaccaca cacaagacac cacagaccac acacaagaca ccacagacca cacacaagac
      301 accacagacc acacacaaga caccacagac cacacacaag acaccacaga ccacacacaa
      361 gacaccacag accacacaca gcatgcaacc tgttcgtacg tatcgaccgt cgccgtaaac
      421 tgcctgcgac catcattctg cgcgccctga actacaccac agagcagatc ctcgacctgt
      481 tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg
      541 aacgcctgcg tggtgaaacc gcatcttttg acatcgaagc taacggtaaa gtgtacgtag
      601 aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac
      661 tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg
      721 agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta
      781 agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaacgat ctggatcacg
      841 gcccatatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg
      901 tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gctgaaagcc
      961 tgttcgagaa cctgttcttc tccgaagacc gttatgactt gtctgcggtt ggtcgtatga
     1021 agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg
     1081 acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg
     1141 atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt
     1201 tccgcgttgg cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc
     1261 tggataccct gatgccacag gatatgatca acgccaagcc gatttccgca gcagtgaaag
     1321 agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga
     1381 ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag
     1441 gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc
     1501 ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat
     1561 acggcttcct tgagactccg tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc
     1621 actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacttgg
     1681 atgaagaagg ccacttcgta gaagacctgg taacttgccg tagcaaaggc gaatccagct
     1741 tgttcagccg cgaccaggtt gactacatgg acgtatccac ccagcaggtg gtatccgtcg
     1801 gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga
     1861 acatgcaacg tcaggccgtt ccgactctgc gcgctgataa gccgctggtt ggtactggta
     1921 tggaacgtgc tgttgccgtt gactccggtg taactgcggt agctaaacgt ggtggtgtcg
     1981 ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg
     2041 gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct
     2101 gtatcaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg
     2161 cagacggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatc gcttactacc
     2221 gattccgcct agttggtcac ttcgacgtat cgtctggaac tccaaccatc gcaggcagag
     2281 aggtctgcaa aatgcaatcc cgaaacagtt cgcaggtaat agttagagcc tgcataacgg
     2341 tttcgggatt ttttatatct gcacaacagg taagagcatt gagtcgataa tcgacacagg
     2401 aaacacatat gaccatgatt acgaatttcg acctgcagcc aagcttggca ctggccgtcg
     2461 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2521 atcccccttt cgccag
//
