LOCUS       sequence-124721-        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  artificial transcription template ""pause ablation"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Harden TT, Wells CD, Friedman LJ, Landick R, Hochschild A, Kondev J,
            Gelles J
  TITLE     Bacterial RNA polymerase can retain sigma70 throughout 
            transcription.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 5. pii: 201513899.
  PUBMED    26733675
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 5. pii: 201513899."
COMMENT     Sequence Label: pCDW115
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..27
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2399..2416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2399..2415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2408..2430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattccag acaacatttt
       61 gaatgcggtc acacgttagc agcatgattg ccacggatgg caacatatta acggcatgat
      121 attgacttat tgaataaaat tgggtaaatt tgactcagcg aggggttaat tcgctcggat
      181 ccagacacca cagaccacac acaagacacc acagaccaca cacaagacac cacagaccac
      241 acacaagaca ccacagacca cacacaagac accacagacc acacacaaga caccacagac
      301 cacacacaag acaccacaga ccacacacag catgcaacct gttcgtacgt atcgaccgtc
      361 gccgtaaact gcctgcgacc atcattctgc gcgccctgaa ctacaccaca gagcagatcc
      421 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
      481 tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag
      541 tgtacgtaga aaaaggccgc cgtatcactg cgcgccacat tcgccagctg gaaaaagacg
      601 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
      661 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
      721 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
      781 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
      841 gcgcactggt agaaatctac cgcatgatgc gccctggcga gccgccgact cgtgaagcag
      901 ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ttatgacttg tctgcggttg
      961 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     1021 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     1081 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg
     1141 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     1201 tgggcgatct ggataccctg atgccacagg atatgatcaa cgccaagccg atttccgcag
     1261 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     1321 tgtctgagat tacgcacaaa cgtcgtatct ccgcactcgg cccaggcggt ctgacccgtg
     1381 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     1441 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc tctgtccgtg tacgcacaga
     1501 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     1561 acgaaattca ctacctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     1621 ccaacttgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     1681 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     1741 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     1801 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg cgctgataag ccgctggttg
     1861 gtactggtat ggaacgtgct gttgccgttg actccggtgt aactgcggta gctaaacgtg
     1921 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     1981 tgtatccggg tgaagcaggt atcgacatct acaacctgac caaatacacc cgttctaacc
     2041 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggtt gaacgtggcg
     2101 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatcg
     2161 cttactaccg attccgccta gttggtcact tcgacgtatc gtctggaact ccaaccatcg
     2221 caggcagaga ggtctgcaaa atgcaatccc gaaacagttc gcaggtaata gttagagcct
     2281 gcataacggt ttcgggattt tttatatctg cacaacaggt aagagcattg agtcgataat
     2341 cgacacagga aacacatatg accatgatta cgaatttcga cctgcagcca agcttggcac
     2401 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2461 ttgcagcaca tccccctttc gccag
//
