LOCUS       sequence-124728-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR gRNA expression vector with an improved scaffold and puro/BFP
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..771)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(772..825)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
       61 agcggccgct caattaagct tgtgccccag tttgctaggg aggtcgcagt atctggccac
      121 tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc tttccagtct
      181 gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct tggatctata
      241 tgtggtcttg atgtttgcga tcagatggct cccgcccacg agcttcaggg ccatgtcgtt
      301 tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct cccagccgag
      361 tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga tcttgacgtt
      421 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
      481 ctcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct tgaagaagtc
      541 ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc tagtagccag
      601 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt
      661 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt tgtccacggt
      721 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca ttgggccagg
      781 attctcctcc acgtcaccgc atgttaggag acttcctctg ccctctcctc cggacccgcc
      841 gccggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc
      901 ggcggtgacg gtgaagccga gccg
//
