LOCUS       sequence-124749-        4077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..765
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtgt ctaagggcga agagctgatt
       61 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
      121 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      181 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
      241 agcagaacct tcatcaacca cacccagggc atccccgatt tctttaagca gtccttccct
      301 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      361 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      421 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag
      481 atgctgtacc ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg
      541 ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa acccgctaag
      601 aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc
      661 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
      721 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtccgg actcagatct
      781 cgagctcaag cttcgaattc gggaaactcg agaacaaagc ttgaaaacgg ccgcatgcca
      841 gtgtttcata cgcgcacgat cgagagcatc ctggagccgg tggcacagca gatctcccac
      901 ctggtgataa tgcacgagga gggcgaggtg gacggcaaag ccattcctga cctcaccgcg
      961 cccgtggccg ccgtgcaggc ggccgtcagc aacctcgtcc gggttggaaa agagactgtt
     1021 caaaccactg aggatcagat tttgaagaga gatatgccac cagcatttat taaggttgag
     1081 aatgcttgca ccaagcttgt ccaggcagct cagatgcttc agtcagaccc ttactcagtg
     1141 cctgctcgag attatctaat tgatgggtca aggggcatcc tctctggaac atcagacctg
     1201 ctccttacct tcgatgaggc tgaggtccgt aaaattatta gagtttgcaa aggaattttg
     1261 gaatatctta cagtggcaga ggtggtggag actatggaag atttggtcac ttacacaaag
     1321 aatcttgggc caggaatgac taagatggcc aagatgattg acgagagaca gcaggagctc
     1381 actcaccagg agcaccgagt gatgttggtg aactcgatga acaccgtgaa agagttgctg
     1441 ccagttctca tttcagctat gaagattttt gtaacaacta aaaactcaaa aaaccaaggc
     1501 atagaggaag ctttaaaaaa tcgcaatttt actctagaaa aaatgagtgc tgaaattaat
     1561 gagataattc gtgtgttaca actcacctct tgggatgaag atgcctgggc cagcaaggac
     1621 actgaagcca tgaagagagc attggcctcc atagactcca aactgaacca ggccaaaggt
     1681 tggctccgtg accctagtgc ctccccaggg gatgctggtg agcaggccat cagacagatc
     1741 ttagatgaag ctggaaaagt tggtgaactc tgtgcaggca aagaacgcag ggagattctg
     1801 ggaacttgca aaatgctagg gcagatgact gatcaagtgg ctgacctccg tgccagagga
     1861 caaggatcct caccggtggc catgcagaaa gctcagcagg tatctcaggg tctggatgtg
     1921 ctcacagcaa aagtggaaaa tgcagctcgc aagctggaag ccatgaccaa ctcaaagcag
     1981 agcattgcaa agaagatcga tgctgctcag aactggcttg cagatccaaa tggtggaccg
     2041 gaaggagaag agcagattcg aggtgctttg gctgaagctc ggaaaatagc agaattatgt
     2101 gatgatccta aagaaagaga tgacattcta cgttcccttg gggaaatatc tgctctgact
     2161 tctaaattag cagatctacg aagacagggg aaaggagatt ctccagaggc tcgagccttg
     2221 gccaaacagg tggccacggc cctgcagaac ctgcagacca aaaccaaccg ggctgtggcc
     2281 aacagcagac cggccaaagc agctgtacac cttgagggca agattgagca agcacagcgg
     2341 tggattgata atcccacagt ggatgaccgt ggagtcggtc aggctgccat ccgggggctt
     2401 gtggccgaag ggcatcgtct ggctaatgtt atgatggggc cttatcggca agatcttctc
     2461 gccaagtgtg accgagtgga ccagctgaca gcccagctgg ctgacctggc tgccagaggg
     2521 gaaggggaga gtcctcaggc acgagcactt gcatctcagc tccaagactc cttaaaggat
     2581 ctaaaagctc ggatgcagga ggccatgact caggaagtgt cagatgtttt cagcgatacc
     2641 acaactccca tcaagctgtt ggcagtggca gccacggcgc ctcctgatgc gcctaacagg
     2701 gaagaggtat ttgatgagag ggcagctaac tttgaaaacc attcaggaaa gcttggtgct
     2761 acggccgaga aggcggctgc ggttggtact gctaataaat caacagtgga aggcattcag
     2821 gcctcagtga agacggcccg agaactcaca ccccaggtgg tctcggctgc tcgtatctta
     2881 cttaggaacc ctggaaatca agctgcttat gaacattttg agaccatgaa gaaccagtgg
     2941 atcgataatg ttgaaaaaat gacagggctg gtggacgaag ccattgatac caaatctctg
     3001 ttggatgctt cagaagaagc aattaaaaaa gacctggaca agtgcaaggt agctatggcc
     3061 aacattcagc ctcagatgct ggttgctggg gcaaccagta ttgctcgtcg ggccaaccgg
     3121 atcctgctgg tggctaagag ggaggtggag aattccgagg atcccaagtt ccgtgaggct
     3181 gtgaaagctg cctctgatga attgagcaaa accatctccc cgatggtgat ggatgcaaaa
     3241 gctgtggctg gaaacatttc cgaccctgga ctgcaaaaga gcttcctgga ctcaggatat
     3301 cggatcctgg gagctgtggc caaggtcaga gaagccttcc aacctcagga gcctgacttc
     3361 ccgccgcctc caccagacct tgaacaactc cgactaacag atgagcttgc tcctcccaaa
     3421 ccacctctgc ctgaaggtga ggtccctcca cctaggcctc caccaccaga ggaaaaggat
     3481 gaagagttcc ctgagcagaa ggccggggag gtgattaacc agccaatgat gatggctgcc
     3541 agacagctcc atgatgaagc tcgcaaatgg tccagcaagg gcaatgacat cattgcagca
     3601 gccaagcgca tggctctgct gatggctgag atgtctcggc tggtaagagg gggcagtggt
     3661 accaagcggg cactcattca gtgtgccaag gacatcgcca aggcctcaga tgaggtgact
     3721 cggttggcca aggaggttgc caagcagtgc acagataaac ggattagaac caacctctta
     3781 caggtatgtg agcgaatccc aaccataagc acccagctca aaatcctgtc cacagtgaag
     3841 gccaccatgc tgggccggac caacatcagt gatgaggagt ctgagcaggc cacagagatg
     3901 ctggttcaca atgcccagaa cctcatgcag tctgtgaagg agactgtgcg ggaagctgaa
     3961 gctgcttcaa tcaaaattcg aacagatgct ggatttacac tgcgctgggt tagaaagact
     4021 ccctggtacc agtaggcggc cgcgactcta gatcataatc agccatacca cattgag
//
