LOCUS       sequence-124754-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9 fused with the Blasticidin 
            resistant gene at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.09"; journalName: "Cell Rep"; date: 
            "2016-10-18- 18"; volume: "17"; issue: "4"; pages: "1193-1205"
COMMENT     Sequence Label: pKLV2-EF1a-BsdCas9-W
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..96)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgagcg gccgcttact ttttcttttt
       61 tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct gagacaggtc
      121 gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt ccagcacctc
      181 tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga aggcggcagg
      241 ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct ctctgatggg
      301 cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt cggccaggat
      361 cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt gcttgtgctg
      421 ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct tctcatagtg
      481 gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt ttcccttctg
      541 cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga acagggagta
      601 cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg cttccagaaa
      661 gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca gcagctcttt
      721 cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca gcacagaata
      781 ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct tctttctggc
      841 gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc cgcctgtctg
      901 cacctcggtc tttttcacga tattcacttg gggcatgctc agcactttcc gcacg
//
