LOCUS       sequence-124756-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu
            LJ, Wolfe SA
  TITLE     DNA-binding-domain fusions enhance the targeting range and precision
            of Cas9.
  JOURNAL   Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
  PUBMED    26480473
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624."
COMMENT     Sequence Label: pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgattcgaa ttcaaggatc aacaagtttg tacaaaaaag caggctggcg ccaccatgga
       61 caagaagtac tccattgggc tcgatatcgg taccaacagc gtcggctggg ccgtcattac
      121 ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag
      181 cataaagaag aacctcattg gagccctcct gttcgactcc ggggagacgg ccgaagccac
      241 gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct
      301 gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga
      361 ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat
      421 cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct
      481 ggtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcacatgat
      541 caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga
      601 caaactcttt atccaactgg ttcagactta caatcagctt ttcgaggaga acccgatcaa
      661 cgcatccggc gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct
      721 cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat
      781 cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc
      841 caag
//
